Add the following volumes to each well in order, according to sample input:

Order of AdditionReagentVol for Sample Input < or = 100ngVol for Sample Input > 100ng
1

RSB

2.5 uL

0 uL

2

RNA Index Anchors

2.5 uL

5 uL

3

LIGX

2.5 uL

2.5 uL