Add the following volumes to each well in order, according to sample input:

Order of AdditionReagentVol for Sample Input < or = 100ngVol for Sample Input > 100ng
1
RSB
2.5 uL
0 uL
2
RNA Index Anchors
2.5 uL
5 uL
3
LIGX
2.5 uL
2.5 uL