| Desired Loading Concentration pM | ul HT1 | ul 20pM Library Pool | ul 20pM PhiX for 1% Spike-In | ul 20pM PhiX for 5% Spike-In | ul 20pM PhiX for 20% Spike-In |
|---|---|---|---|---|---|
1.3 | 1215.5 | 84.5 | 0.85 | 4.23 | 16.90 |
1.4 | 1209.0 | 91.0 | 0.91 | 4.55 | 18.20 |
1.5 | 1202.5 | 97.5 | 0.98 | 4.88 | 19.50 |
1.6 | 1196.0 | 104.0 | 1.04 | 5.20 | 20.80 |
1.7 | 1189.5 | 110.5 | 1.11 | 5.53 | 22.10 |
1.8 | 1183.0 | 117.0 | 1.17 | 5.85 | 23.40 |