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NextSeq 1000/2000 Library Kits (Full View)

Library TypeFinal Loading Conc. (pM)2 nM Library Volume (µL)RSB with Tween 20 Volume (µL)Index KitIndex LengthPhiX SpikeNotes
Ampliseq for Illumina Library PLUS
750
9
15
Illumina DNA Prep
750
9
15
IDT-ILMN Nextera UD Index Set _ for Nextera DNA Flex
10 bp dual
1 µL 1 nM Phix (5% PhiX)
Illumina DNA Prep with Enrichment
1000
12
12
Illumina Stranded Total RNA with Ribo-Zero Plus
750
9
15
IDT-ILMN Nextera UD Index Set _ for Nextera DNA Flex
10 bp dual
1 µL 1 nM Phix (5% PhiX)
Illumina Stranded mRNA Prep
750
9
15
IDT-ILMN Nextera UD Index Set _ for Nextera DNA Flex
10 bp dual
1 µL 1 nM Phix (5% PhiX)
Illumina DNA PCR-Free
1000
12
12
TruSeq DNA Nano 350
1200
14.4
9.6
TruSeq DNA Nano 550
1500
18
6
TruSeq Stranded mRNA
1000
12
12
IDT-ILMN TruSeq DNA-RNA UD 96 Indexes
8 bp dual
1 µL 1 nM Phix (4% PhiX)
100% PhiX
650
7.8
16.2
10X Chromium Next GEM 3’ Gene Expression Kit v3.1 (also for Multiome Gene Expression)
650
7.8
16.2
Single Cell RNA Index-Adapters 1-B (Dual Index Kit TT Set A)
8 bp single OR 10 bp dual
1 µL 1 nM Phix (6% PhiX)
10X Chromium Next GEM Single Cell ATAC
Single Cell ATAC Index-Adapters 1 (Single Index Kit N Set A)
10X Chromium Next GEM Single Cell Multiome ATAC
650
7.8
16.2
Single Cell ATAC Index-Adapters 2 (Single Index Kit N Set A)
8 bp single
1 µL 1 nM Phix (6% PhiX)
Takara SMART Seq HT PLUS Kit
1000
12
12
IDT-ILMN TruSeq DNA-RNA UD 96 Indexes
8 bp dual
1 µL 1 nM Phix (4% PhiX)
Takara Pico v3 Kit
1000
12
12
IDT-ILMN TruSeq DNA-RNA UD 96 Indexes
8 bp dual
2.75 µL 1 nM PhiX (10% for a patterned flow cell) > 2.75 µL PhiX in total of 26.75 µL
For more information, check Appendices B or C in their User Manual
NEB Libraries