Prepare the following:

NAME
LOCATION
ACTION
Anchor Plate

-20°C Freezer

Thaw at RT, vortex, centrifuge briefly

Index Adapter Plate

-20°C Freezer

Thaw at RT, vortex, centrifuge for 1 minute

A-Tailing Mix (ATL4)

-20°C Freezer (Ligation box)

Thaw at RT, flick to mix, centrifuge briefly

Stop Ligation Buffer (STL)

-20°C Freezer (Ligation box, red cap)

Thaw at RT, vortex, centrifuge briefly

Ampure XP Beads/Homemade beads

4°C Fridge

Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use

Resuspension Buffer (RSB)

-20°C Freezer (Ligation box)

Thaw at RT, vortex briefly

Enhanced PCR Mix (EPM)

-20°C Freezer (Ligation box)

Thaw at RT, invert to mix, centrifuge briefly

Ligation Mix (LIGX)

-20°C Freezer (Ligation box)

Store until needed, flick to mix, centrifuge briefly