Prepare the following:

NAME
LOCATION
ACTION
Ampure XP Beads/Homemade Beads

4°C Fridge

Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use

Resuspension Buffer (RSB)

-20°C Freezer (cDNA synthesis box)

Thaw at RT, vortex briefly

Reverse Transcriptase (RVT)

-20°C Freezer (cDNA synthesis box)

Store until needed. Flick to mix, centrifuge briefly

Second Strand Marking Mix (SMM)

-20°C Freezer (cDNA synthesis box)

Thaw on ice, invert to mix, centrifuge briefly