| Desired Loading Concentration pM | ul Hyb Buffer | ul 5pM Library Pool | ul 20pM PhiX for 1% Spike-In | ul 20pM PhiX for 5% Spike-In | ul 20pM PhiX for 20% Spike-In |
|---|---|---|---|---|---|
1.3 | 370 | 130 | 0.325 | 1.63 | 6.5 |
1.4 | 360 | 140 | 0.350 | 1.75 | 7.0 |
1.5 | 350 | 150 | 0.375 | 1.88 | 7.5 |
1.6 | 340 | 160 | 0.400 | 2.00 | 8.0 |
1.7 | 330 | 170 | 0.425 | 2.13 | 8.5 |
1.8 | 320 | 180 | 0.450 | 2.25 | 9.0 |