Prepare the following:

NameLocationAction
One-step Buffer

-20°C freezer

Thaw at room temperature

10X Reaction Buffer

-20°C Freezer

If made, thaw on ice; if not made, see below

Nuclease-free Water

-20°C freezer

Thaw at room temperature

3’ SMARTSeq CDS Primer II

-20°C Freezer

Thaw on ice

SMART-Seq HT Oligonucleotide

-20°C freezer

Thaw on ice

RNase Inhibitor (RRI)

-20°C freezer

Thaw on ice

Elution Buffer

-20°C freezer

Thaw at room temperature

SeqAmp DNA Polymerase

-20°C freezer

Store until needed

SMARTScribe Reverse Transcriptase

-20°C freezer

Store until needed