Volumes for Digestion Mix

1000 cells2000 cells3000 cells
ddH2O

39.5 uL

79 uL

118.5 uL

10X Fast Digest Buffer

4.5 uL

9 uL

13.5 uL

Exonuclease I

2.5 uL

5 uL

7.5 uL

HinFI

3.5 uL

7 uL

10.5 uL

Total volume

50 uL

100 uL

150 uL