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Protocols

2nd strand master mix

ComponentVolume per rxn
Nuclease-free water
12 uL
NEB T7 High Yield 10X Buffer
8 uL
NTP [4 different NTP buffers, ATP, UTP, CTP, GTP] (100mM)
8 uL each
NEB T7 High Yield Enzyme Mix
8 uL
60 uL