Kit reagent | Volume per rxn (uL) |
|---|---|
SMART TSO Mix v2/v3 (very viscous, mix well) | 4.5 |
RNase Inhibitor | 0.5 |
SMARTScribe Reverse Transcriptase | 2.0 |
5x First-Strand Buffer | 4.0 |
**Total Volume per reaction | 11.0** |
Kit reagent | Volume per rxn (uL) |
|---|---|
SMART TSO Mix v2/v3 (very viscous, mix well) | 4.5 |
RNase Inhibitor | 0.5 |
SMARTScribe Reverse Transcriptase | 2.0 |
5x First-Strand Buffer | 4.0 |
**Total Volume per reaction | 11.0** |