PCR reaction master mix

NameVolume (uL)Comments
Transposed DNA
10
Nuclease-free water
12.7
Illumina Primer 1
1
Illumina Primer 2
1
100x SYBR Green I
0.3
10,000x SYBR Green I is diluted in EB Buffer to make a 100x working solution.
NEBNext High-Fidelity 2x PCR Master Mix
25
5o uL total