| Name | Volume (uL) | Comments |
|---|---|---|
Transposed DNA | 10 | |
Nuclease-free water | 12.7 | |
Illumina Primer 1 | 1 | |
Illumina Primer 2 | 1 | |
100x SYBR Green I | 0.3 | 10,000x SYBR Green I is diluted in EB Buffer to make a 100x working solution. |
NEBNext High-Fidelity 2x PCR Master Mix | 25 | |
5o uL total |