RT master mix recipe

ComponentVolume/ rxnVolume for 2 rxnsVolume for 4 rxns
RT Reagent B
20.68 uL
41.36
82.72
Template Switch Oligo
2.64 uL
5.28
10.56
Reducing Agent B I
2.2 uL
4.4
8.8
RT Enzyme C
9.57 uL
19.14
38.28
Total 35.09 uL