RT master mix recipe

Component
Volume/ rxn
Volume for 2 rxns
Volume for 4 rxns
RT Reagent B

20.68 uL

41.36

82.72

Template Switch Oligo

2.64 uL

5.28

10.56

Reducing Agent B I

2.2 uL

4.4

8.8

RT Enzyme C

9.57 uL

19.14

38.28

Total 35.09 uL