NAME | LOCATION | ACTION | VOLUME |
|---|---|---|---|
Ampure XP Beads/Homemade beads | 4°C Fridge | Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use | 174uL per sample for entire prep |
RNAClean XP Beads (Ampure beads can also be used, but RNAClean are certified free of RNases) | 4°C Fridge | Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use | 60 uL per sample for entire prep |
Fresh 80% Ethanol (200 proof EtOH + MilliQ water) | RT Bench | Mix fresh each day. Low% EtOH negatively affects cleanups. | 1.6mL per sample for entire prep |
RNAse Free RT-PCR Grade Water | -20°C Freezer | Thaw at RT | As needed for diluting RNA input |
Magnetic Stand for 96 well plates | RT Bench |