Prepare the following non-Illumina materials:

NAMELOCATIONACTIONVOLUME
Ampure XP Beads/Homemade beads
4°C Fridge
Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use
174uL per sample for entire prep
RNAClean XP Beads (Ampure beads can also be used, but RNAClean are certified free of RNases)
4°C Fridge
Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use
60 uL per sample for entire prep
Fresh 80% Ethanol (200 proof EtOH + MilliQ water)
RT Bench
Mix fresh each day. Low% EtOH negatively affects cleanups.
1.6mL per sample for entire prep
RNAse Free RT-PCR Grade Water
-20°C Freezer
Thaw at RT
As needed for diluting RNA input
Magnetic Stand for 96 well plates
RT Bench