Prepare the following Illumina reagents:

NAMELOCATIONACTION
Elution Buffer (ELB)
-20C Freezer (Ribo Zero Plus box, clear cap)
Thaw at RT, vortex and centrifuge briefly
Depletion Probe Buffer (DB1)
-20C Freezer (Ribo Zero Plus box, blue cap)
Thaw at RT, vortex and centrifuge briefly
Depletion Probe Pool (DP1)
-20C Freezer (Ribo Zero Plus box, blue cap)
Thaw at RT, vortex and centrifuge briefly
RNA Depletion Buffer (RDB)
-20C Freezer (Ribo Zero Plus box, yellow cap)
Thaw and keep on ice, vortex briefly before use
RNA Depletion Enzyme (RDE)
-20C Freezer (Ribo Zero Plus box, yellow cap)
Store until needed, flick to mix, centrifuge briefly
Probe Removal Buffer (PRB)
-20C Freezer (Ribo Zero Plus box, red cap)
Thaw at RT, vortex and centrifuge briefly
Probe Removal Enzyme (PRE)
-20C Freezer (Ribo Zero Plus box, red cap)
Store until needed, flick to mix, centrifuge briefly