Prepare the following Illumina reagents:

NAMELOCATIONACTION
Elution Buffer (ELB)

-20°C Freezer (Ribo Zero Plus box, clear cap)

Thaw at RT, vortex and centrifuge briefly

Depletion Probe Buffer (DB1)

-20°C Freezer (Ribo Zero Plus box, blue cap)

Thaw at RT, vortex and centrifuge briefly

Depletion Probe Pool (DP1)

-20°C Freezer (Ribo Zero Plus box, blue cap)

Thaw at RT, vortex and centrifuge briefly

Depletion Probe Microbiome (DPM) (for microbiome kit ONLY)

-20°C Freezer (alone in its own box)

Thaw at RT, vortex and centrifuge briefly

RNA Depletion Buffer (RDB)

-20°C Freezer (Ribo Zero Plus box, yellow cap)

Thaw and keep on ice, vortex briefly before use

RNA Depletion Enzyme (RDE)

-20°C Freezer (Ribo Zero Plus box, yellow cap)

Store until needed, flick to mix, centrifuge briefly

Probe Removal Buffer (PRB)

-20°C Freezer (Ribo Zero Plus box, red cap)

Thaw at RT, vortex and centrifuge briefly

Probe Removal Enzyme (PRE)

-20°C Freezer (Ribo Zero Plus box, red cap)

Store until needed, flick to mix, centrifuge briefly