Prepare the following non-Illumina materials:

Ampure XP Beads/Homemade XP Beads

4°C Fridge

Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use

174uL per sample for entire prep

Fresh 80% Ethanol (200 proof EtOH + MilliQ water)

RT Bench

Mix fresh each day. Low% EtOH negatively affects cleanups.

1.2mL per sample for entire prep

RNAse Free RT-PCR Grade Water

-20°C Freezer

Thaw at RT

As needed for diluting RNA input

Post-Style Magnetic Stand for 96 well plates

RT Bench