Prepare the following non-Illumina materials:

NAMELOCATIONACTIONVOLUME
Ampure XP Beads/Homemade XP Beads
4°C Fridge
Let stand at RT for 30 minutes to bring to RT, vortex and invert thoroughly before use
174uL per sample for entire prep
Fresh 80% Ethanol (200 proof EtOH + MilliQ water)
RT Bench
Mix fresh each day. Low% EtOH negatively affects cleanups.
1.2mL per sample for entire prep
RNAse Free RT-PCR Grade Water
-20°C Freezer
Thaw at RT
As needed for diluting RNA input
Post-Style Magnetic Stand for 96 well plates
RT Bench