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NameSupplier and cat#FluorophoreLocationComments
Ulex Europaeus Agglutinin I (UEA I)
Vector labs; cat# FL-1061
Fluorescein (FITC)
4ºC antibody box
Used to detect mucins
Rabbit anti-human zona occludens-1 (ZO-1; mAb clone D6L1E)
Cell Signaling Technology; cat# 13663S
None; requires secondary Ab
-20C antibody box
Used to detect tight junctions
Mouse anti-human Ezrin (clone 4A5)
Millipore/Sigma; cat# MAB3822-C
None; requires secondary Ab
4ºC antibody box
Used to detect apical brush border
Phalloidin
Invitrogen; cat# A12380
Alexa 568
Toxin that bind competitively to the same sites in F actin
Goat anti-rabbit secondary antibody
Invitrogen; cat# A-11036
Alexa 568
We use with ZO-1 primary
Goat anti-mouse secondary antibody
Invitrogen; cat# A-11031
Alexa 568
4ºC antibody box
We use with Ezrin primary
Goat anti-mouse secondary antibody
Invitrogen; cat# A-21036
Alexa 700
4ºC antibody box
We use with Ezrin primary
Goat anti-mouse secondary antibody
Invitrogen; cat# A-32728
Alexa 647
We use with Ezrin primary
VectaShield Vibrance Antifade Mounting medium with DAPI
Vector Labs; Cat# H-1800-10
DAPI
4ºC antibody box
Counterstain for nuclei
GFP-expressing Citrobacter rodentium (Chloramphenicol resistance)
Provided by Gabriel Nunez' lab
GFP
-80ºC
WT Citrobacter rodentium (Kanamycin resistance)
Provided by Gabriel Nunez' lab
None; requires secondary Ab
-80ºC