Name | Supplier and cat# | Fluorophore | Location | Comments |
|---|---|---|---|---|
Ulex Europaeus Agglutinin I (UEA I) | Vector labs; cat# FL-1061 | Fluorescein (FITC) | 4ºC antibody box | Used to detect mucins |
Rabbit anti-human zona occludens-1 (ZO-1; mAb clone D6L1E) | Cell Signaling Technology; cat# 13663S | None; requires secondary Ab | -20C antibody box | Used to detect tight junctions |
Mouse anti-human Ezrin (clone 4A5) | Millipore/Sigma; cat# MAB3822-C | None; requires secondary Ab | 4ºC antibody box | Used to detect apical brush border |
Phalloidin | Invitrogen; cat# A12380 | Alexa 568 | Toxin that bind competitively to the same sites in F actin | |
Goat anti-rabbit secondary antibody | Invitrogen; cat# A-11036 | Alexa 568 | We use with ZO-1 primary | |
Goat anti-mouse secondary antibody | Invitrogen; cat# A-11031 | Alexa 568 | 4ºC antibody box | We use with Ezrin primary |
Goat anti-mouse secondary antibody | Invitrogen; cat# A-21036 | Alexa 700 | 4ºC antibody box | We use with Ezrin primary |
Goat anti-mouse secondary antibody | Invitrogen; cat# A-32728 | Alexa 647 | We use with Ezrin primary | |
VectaShield Vibrance Antifade Mounting medium with DAPI | Vector Labs; Cat# H-1800-10 | DAPI | 4ºC antibody box | Counterstain for nuclei |
GFP-expressing Citrobacter rodentium (Chloramphenicol resistance) | Provided by Gabriel Nunez' lab | GFP | -80ºC | |
WT Citrobacter rodentium (Kanamycin resistance) | Provided by Gabriel Nunez' lab | None; requires secondary Ab | -80ºC |