Non-Illumina Reagents

RNase-free water

-20C Freezer (on the door)

Thaw at room temp and use for dilutions

80% Ethanol


mix 200 proof EtOH and Milli-Q water to make fresh 80% EtOH each day

Superscript II (invitrogen)

-20C Freezer

Thaw on ice and mix (at appropriate ratio) with FSA

AMPure XP Beads

4C Fridge

Thaw at room temp for 30 min then vortex vigorously