Reagent | Location | Action |
---|---|---|
RNase-free water | -20C Freezer (on the door) | Thaw at room temp and use for dilutions |
80% Ethanol | Bench | mix 200 proof EtOH and Milli-Q water to make fresh 80% EtOH each day |
Superscript II (invitrogen) | -20C Freezer | Thaw on ice and mix (at appropriate ratio) with FSA |
AMPure XP Beads | 4C Fridge | Thaw at room temp for 30 min then vortex vigorously |