Non-Illumina Reagents

ReagentLocationAction
RNase-free water
-20C Freezer (on the door)
Thaw at room temp and use for dilutions
80% Ethanol
Bench
mix 200 proof EtOH and Milli-Q water to make fresh 80% EtOH each day
Superscript II (invitrogen)
-20C Freezer
Thaw on ice and mix (at appropriate ratio) with FSA
AMPure XP Beads
4C Fridge
Thaw at room temp for 30 min then vortex vigorously