Illumina Reagents

NameLocationBox NameAction
RNA Purification Beads (oligo dT beads)
4C Fridge
TruSeq Stranded mRNA LP Box 1
Thaw 30 min at room temp then vortex vigorously
Bead Washing Buffer
Aliquot from -20C freezer, then store in 4C fridge
TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots)
Thaw at room temp
Elution Buffer
Aliquot from -20C freezer, then store in 4C fridge
TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots)
Thaw at room temp
Bead Binding Buffer
Aliquot from -20C freezer, then store in 4C fridge
TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots)
Thaw at room temp
Fragment Prime Finish Mix
-20C Freezer
TruSeq Stranded mRNA LP Box 2
Thaw at room temp
First Strand Synthesis (FSA)
-20C Freezer
TruSeq Stranded RNA Core LP Box 2 (brown tube)
Thaw at room temp then combine with Superscript II
Second Strand Marking Mix (SMM)
-20C Freezer
TruSeq Stranded RNA Core LP Box 2
Thaw at room temp
Resuspendion Buffer
Aliquot from -20C freezer, then store in 4C fridge
TruSeq Strnd RNA Core LP Box 1
Thaw at room temp