Name | Location | Box Name | Action |
|---|---|---|---|
RNA Purification Beads (oligo dT beads) | 4C Fridge | TruSeq Stranded mRNA LP Box 1 | Thaw 30 min at room temp then vortex vigorously |
Bead Washing Buffer | Aliquot from -20C freezer, then store in 4C fridge | TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots) | Thaw at room temp |
Elution Buffer | Aliquot from -20C freezer, then store in 4C fridge | TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots) | Thaw at room temp |
Bead Binding Buffer | Aliquot from -20C freezer, then store in 4C fridge | TruSeq Stranded mRNA LP Box 2 (bulk), TruSeq Stranded mRNA LP Box 1 (aliquots) | Thaw at room temp |
Fragment Prime Finish Mix | -20C Freezer | TruSeq Stranded mRNA LP Box 2 | Thaw at room temp |
First Strand Synthesis (FSA) | -20C Freezer | TruSeq Stranded RNA Core LP Box 2 (brown tube) | Thaw at room temp then combine with Superscript II |
Second Strand Marking Mix (SMM) | -20C Freezer | TruSeq Stranded RNA Core LP Box 2 | Thaw at room temp |
Resuspendion Buffer | Aliquot from -20C freezer, then store in 4C fridge | TruSeq Strnd RNA Core LP Box 1 | Thaw at room temp |