| Reagent | Location | Box Name | Action |
|---|---|---|---|
A-tailing mix | -20C Freezer | TruSeq Strnd RNA Core LP Box 1 | Thaw at room temp |
Resuspension buffer | Aliquot from -20C freezer, aliquots stored in 4C fridge | TruSeq Strnd RNA Core LP Box 1 (bulk tube), TruSeq Stranded mRNA LP Box 1 (aliquots) | Thaw at room temp for 30 min (You will need 112.5 µL per sample for Day 2) |
Ligation Mix (LIG) | -20C Freezer | TruSeq Strnd RNA Core LP Box 1 | Store in freezer until immediately before use, and replace immediately after use |
Index Adaptors | -20C Freezer | TruSeq RNA CD Indexes | Thaw at room temp for 10 min, reseal and replace after use |
Stop Ligation Buffer | -20C Freezer | TruSeq Strnd RNA Core LP Box 1 | Thaw at room temp, centrifuge before use |
PCR Primer Cocktail (PPC) | -20C Freezer | TruSeq Stranded RNA Core LP Box 2 | Thaw at room temp, invert to mix, centrifuge (NO VORTEX) |
PCR Master Mix (PMM) | -20C Freezer | TruSeq Stranded RNA Core LP Box 2 | Thaw on ice, invert to mix, centrifuge (NO VORTEX) |