Illumina Reagents

ReagentLocationBox NameAction
A-tailing mix
-20C Freezer
TruSeq Strnd RNA Core LP Box 1
Thaw at room temp
Resuspension buffer
Aliquot from -20C freezer, aliquots stored in 4C fridge
TruSeq Strnd RNA Core LP Box 1 (bulk tube), TruSeq Stranded mRNA LP Box 1 (aliquots)
Thaw at room temp for 30 min (You will need 112.5 µL per sample for Day 2)
Ligation Mix (LIG)
-20C Freezer
TruSeq Strnd RNA Core LP Box 1
Store in freezer until immediately before use, and replace immediately after use
Index Adaptors
-20C Freezer
TruSeq RNA CD Indexes
Thaw at room temp for 10 min, reseal and replace after use
Stop Ligation Buffer
-20C Freezer
TruSeq Strnd RNA Core LP Box 1
Thaw at room temp, centrifuge before use
PCR Primer Cocktail (PPC)
-20C Freezer
TruSeq Stranded RNA Core LP Box 2
Thaw at room temp, invert to mix, centrifuge (NO VORTEX)
PCR Master Mix (PMM)
-20C Freezer
TruSeq Stranded RNA Core LP Box 2
Thaw on ice, invert to mix, centrifuge (NO VORTEX)