The structure and clarity of 10X genomics’ own User Guide is excellent and we highly suggest you simply download and follow along with the latest version of their protocol here.
We have both a Chromium Controller and a Chromium X. The Chromium X can do all that the Chromium Controller can do, as well as all the newer assays. Unless otherwise necessary, use the Chromium X.
Cell Surface Protein
10X Genomics’ cell surface protein protocol is compatible with antibodies and corresponding protocol at Biolegend. Please visit their site to determine which antibodies will best suit your needs.