Chromium Next GEM Single-Cell 3’ Kit v3.1 (Feature Barcoding)
Protocol
The structure and clarity of the 10X Genomics User Guide is excellent and we highly suggest you simply download and follow along with the latest version of their protocol here.
Instrumentation
We have both a Chromium Controller and a Chromium X. The Chromium X can do all that the Chromium Controller can do, as well as all the newer assays. Unless otherwise necessary, use the Chromium X.
Cell Surface Protein
10X Genomics’ cell surface protein protocol is compatible with antibodies and the corresponding protocol at Biolegend. Please visit their site to determine which antibodies will best suit your needs.