Usage: squid [options] -b <Input_BAM> -o <Output_Prefix>
Options:
--help
--version
Extra input options:
--bwa If input BAM is generated by BWA or SpeedSeq aligner (default assuming STAR aligner)
-c string Chimeric BAM alignment (Required if using STAR aligner)
-f string Genome FASTA file
Parsing alignment options:
-pt bool Phred type: 0 for Phred33, 1 for Phred64 (0)
-pl int Maximum Length of low Phred score to filter alignment (10)
-pm int Low Phred score threshold (4)
-mq int Mapping quality to filter alignment (1)
Constructing graph options:
-dp int Maximum distance of aligning positions for concordant alignment (50000)
-di int Maximum distance of segment indexes for concordant alignment (20)
-w int Minimum edge weight (5)
-r double Discordant edge ratio multiplier / normal tumor cell ratio (8)
-a int Max allowed degree (5)
Output options:
-G bool Output gragh file (0)
-CO bool Output ordering of connected components (0)
-TO bool Output ordering of all segments (0)
-RG bool Output rearranged genome sequence (0)
separate chimeric alignment BAM file is required if using STAR aligner.